Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine
Zhenhao Zhang, Jingli Yan, Zhengping Li
Abstract
The peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) is applied for the first time to the sensitive detection of locus-specific 5-hydroxymethylcytosine (5hmC) in DNA with low non-specific amplification. This strategy greatly shortens reaction time, improves sensitivity, and reduces non-specific amplification.
Topics & Concepts
Bisulfite5-HydroxymethylcytosineChemistryLocus (genetics)BiochemistryGeneDNA methylationGene expressionEpigenetics and DNA MethylationFolate and B Vitamins ResearchHIV Research and Treatment