What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses
Janina Breuer, Patrick Barth, Yannic Noe, Lyudmila Shalamova, Alexander Goesmann, Friedemann Weber, Oliver Roßbach
Abstract
transcribed RNA in coronavirus disease 2019 (COVID-19) vaccines, the question arose whether therapeutic circular RNAs trigger cellular antiviral defense mechanisms when delivered systemically. In this study, we present data on the cellular innate immune response as a consequence of liposome-based transfection of the circular RNA sponges we previously used to inhibit microRNA function. We find that circular RNAs produced by the presented methodology do not trigger the antiviral response and do not activate innate immune-signaling pathways.
Topics & Concepts
Circular RNAmicroRNARNABiologyInnate immune systemDecoyCell biologyTranscription (linguistics)Immune systemComputational biologyGeneticsGenePhilosophyReceptorLinguisticsCircular RNAs in diseasesMicroRNA in disease regulationRNA Research and Splicing