The food-associated resistome is shaped by processing and production environments
Narciso M. Quijada, José F. Cobo‐Díaz, Vincenzo Valentino, Coral Barcenilla, Francesca De Filippis, Raúl Cabrera‐Rubio, Niccolò Carlino, Federica Pinto, Monika Dzieciol, Inés Calvete‐Torre, Carlos Sabater, Francesco Rubino, Stephen Knobloch, Sigurlaug Skírnisdóttir, Lorena Ruíz, Mercedes López, Miguel Prieto Maradona, V. Marteinsson, Abelardo Margollés, Nicola Segata, Paul D. Cotter, Martin Wagner, Danilo Ercolini, Avelino Álvarez‐Ordóñez
Abstract
Food production systems may act as transmission routes for antimicrobial-resistant (AMR) bacteria and AMR genes (AMRGs) to humans. However, the food resistome remains poorly characterized. Here 1,780 raw-material (milk, brine, fresh meat and so on), end-product (cheese, fish, meat products and vegetables) and surface (processing, cooling, smoking, ripening and packing rooms) samples from 113 food processing facilities were subjected to whole-metagenome sequencing. Assembly-free analyses demonstrated that >70% of all known AMRGs, including many predicted to confer resistance to critically important antibiotics, circulate throughout food production chains, with those conferring resistance to tetracyclines, β-lactams, aminoglycosides and macrolides being the most abundant overall. An assembly-based analysis highlighted that bacteria from the ESKAPEE group, together with Staphylococcus equorum and Acinetobacter johnsonii, were the main AMRG carriers. Further evaluation demonstrated that ~40% of the AMRGs were associated with mobile genetic elements, mainly plasmids. These findings will help guide the appropriate use of biocides and other antimicrobials in food production settings when designing efficient antimicrobial stewardship policies.