Metal-responsive reversible binding of triplex-forming oligonucleotides with 5-hydroxyuracil nucleobases
Kotaro Nishiyama, Keita Mori, Yusuke Takezawa, Mitsuhiko Shionoya
Abstract
Metal-responsive triplex-forming oligonucleotides (TFOs) were synthesised by incorporating 5-hydroxyuracil (UOH) nucleobases as metal recognition sites. Binding of the UOH-containing TFO to the target natural DNA duplexes was reversibly regulated by the addition and removal of GdIII ions under isothermal conditions.
Topics & Concepts
NucleobaseOligonucleotideThymineLigand (biochemistry)ChemistryCombinatorial chemistryMetalScaffoldDNABiochemistryOrganic chemistryReceptorComputer scienceDatabaseDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesSupramolecular Chemistry and Complexes