One-pot isothermal amplification permits recycled activation of CRISPR/Cas12a for sensing terminal deoxynucleotidyl transferase activity
Jianguo Xu, Haidong Yang, Zhuqi Sui, Xinyue Yuan, Lee Jia, Longhua Guo
Abstract
This study introduces a one-pot isothermal amplification assay for ultrasensitive analysis of terminal deoxynucleotidyl transferase (TdT) activity. The system realizes recycled activation of CRISPR/Cas12a, enabling exceptional signal amplification. This approach maximizes the simplicity of the detection method, offering a promising avenue for molecular disease diagnosis.
Topics & Concepts
Terminal deoxynucleotidyl transferaseLoop-mediated isothermal amplificationTransferaseChemistryIsothermal processTUNEL assayCRISPRMolecular biologyBiochemistryBiologyEnzymePhysicsDNAApoptosisGeneThermodynamicsAdvanced biosensing and bioanalysis techniquesAdvanced Nanomaterials in CatalysisBiosensors and Analytical Detection