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Stable-Isotope-Informed, Genome-Resolved Metagenomics Uncovers Potential Cross-Kingdom Interactions in Rhizosphere Soil

Evan Starr, Shengjing Shi, Steven J. Blazewicz, Benjamin J. Koch, Alexander J. Probst, Bruce A. Hungate, Jennifer Pett‐Ridge, Mary K. Firestone, Jillian F. Banfield

2021mSphere73 citationsDOIOpen Access PDF

Abstract

-derived carbon, we demonstrated their vital roles in redistribution of plant-derived C into the soil environment through bacterial cell lysis. The use of stable-isotope probing (SIP) to identify consumption (or lack thereof) of root-derived C by key microbial community members within highly complex microbial communities opens the way for assessing manipulations of bacteria and phage with potentially beneficial and detrimental traits, ultimately providing a path to improved plant health and soil carbon storage.

Topics & Concepts

RhizosphereStable-isotope probingMetagenomicsBiologyGenomeBulk soilSoil microbiologyBotanyBacteriaGeneGeneticsMicroorganismBacteriophages and microbial interactionsGenomics and Phylogenetic StudiesLegume Nitrogen Fixing Symbiosis
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