Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes
Ricardo S Randall, Claire Jourdain, Anna Nowicka, Kateřina Kaduchová, Michaela Kubová, Mohammad A. Ayoub, Veit Schubert, Christophe Tatout, Isabelle Colas, Kalyanikrishna, Sophie Desset, Sarah Mermet, Aurélia Boulaflous-Stevens, Ivona Kubalová, Terezie Mandáková, Stefan Heckmann, Martin A. Lysák, Martina Panatta, Raffaella Santoro, Daniel Schubert, Aleš Pečinka, Devin Routh, Célia Baroux
Abstract
3D FISH: three-dimensional fluorescence in situ hybridization; 3D: three-dimensional; ASY1: ASYNAPTIC 1; CC: chromocenters; CO: Crossover; DAPI: 4',6-diamidino-2-phenylindole; DMC1: DNA MEIOTIC RECOMBINASE 1; DSB: Double-Strand Break; FISH: fluorescence in situ hybridization; GFP: GREEN FLUORESCENT PROTEIN; HEI10: HUMAN ENHANCER OF INVASION 10; NCO: Non-Crossover; NE: Nuclear Envelope; Oligo-FISH: oligonucleotide fluorescence in situ hybridization; RNPII: RNA Polymerase II; SC: Synaptonemal Complex; SIM: structured illumination microscopy; ZMM (ZIP: MSH4: MSH5 and MER3 proteins); ZYP1: ZIPPER-LIKE PROTEIN 1.