Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks
Shinji Sakai, Takashi Kotani, Ryohei Harada, Ryota Goto, Takahiro Morita, Soukaina Bouissil, Pascal Dubessay, Guillaume Pierre, Philippe Michaud, Redouan El Boutachfaiti, Masaki Nakahata, Masaru Kojima, Emmanuel Petit, Cédric Delattre
Abstract
In this present work, we developed a phenol grafted polyglucuronic acid (PGU) and investigated the usefulness in tissue engineering field by using this derivative as a bioink component allowing gelation in extrusion-based 3D bioprinting. The PGU derivative was obtained by conjugating with tyramine, and the aqueous solution of the derivative was curable through a horseradish peroxidase (HRP)-catalyzed reaction. From 2.0 w/v% solution of the derivative containing 5 U/mL HRP, hydrogel constructs were successfully obtained with a good shape fidelity to blueprints. Mouse fibroblasts and human hepatoma cells enclosed in the printed constructs showed about 95% viability the day after printing and survived for 11 days of study without a remarkable decrease in viability. These results demonstrate the great potential of the PGU derivative in tissue engineering field especially as an ink component of extrusion-based 3D bioprinting.