Current advances in microcell-mediated chromosome transfer technology and its applications
Teruhiko Suzuki, Yasuhiro Kazuki, Takahiko Hara, Mitsuo Oshimura
Abstract
Chromosomes and chromosomal gene delivery vectors, human/mouse artificial chromosomes (HACs/MACs), can introduce megabase-order DNA sequences into target cells and are used for applications including gene mapping, gene expression control, gene/cell therapy, and the development of humanized animals and animal models of human disease. Microcell-mediated chromosome transfer (MMCT), which enables chromosome transfer from donor cells to target cells, is a key technology for these applications. In this review, we summarize the principles of gene transfer with HACs/MACs; their engineering, characteristics, and utility; and recent advances in the chromosome transfer technology.
Topics & Concepts
BiologyHuman artificial chromosomeChromosome engineeringGene transferMicrocellChromosomeGeneComputational biologyGeneticsCell biologyComputer scienceOperating systemCRISPR and Genetic EngineeringRNA Interference and Gene DeliveryVirus-based gene therapy research