Rapid Airborne Influenza Virus Quantification Using an Antibody-Based Electrochemical Paper Sensor and Electrostatic Particle Concentrator
Jyoti Bhardwaj, Myeong-Woo Kim, Jaesung Jang
Abstract
via direct protein peroxidation upon exposure of a high electrostatic field. However, minimal losses in antigenicity of NP of the influenza viruses were observed, and the concentration of the H1N1 viruses was more than 160 times higher in the EPC than the BioSampler upon using NP-Ab based paper sensors after 60 min collection. This NP-Ab-based paper sensors with the EPC provided measurements comparable to quantitative polymerase chain reaction (qPCR) but much quicker, specific to the influenza H1N1 viruses in the presence of other airborne microorganisms and beads, and more cost-effective than enzyme-linked immunosorbent assay and qPCR.
Topics & Concepts
AntigenicityVirologyHemagglutinin (influenza)VirusInfluenza A virusChemistryGlutaraldehydeNucleoproteinAntibodyBicinchoninic acid assayBioaerosolBiologyChromatographyAerosolImmunologyOrganic chemistryRespiratory viral infections researchSARS-CoV-2 detection and testingBiosensors and Analytical Detection