When Small becomes Too Big: Expanding the Use of In‐Cell Solid‐State NMR Spectroscopy
Siddarth Narasimhan, Gert E. Folkers, Marc Baldus
Abstract
Solution-state NMR spectroscopy has become a powerful tool to study soluble proteins in cells, provided that they tumble sufficiently fast. In addition, cryo-electron tomography (cryo-ET) has recently displayed a tremendous potential to probe structures of large proteins and assemblies in their native cellular environments. However, challenges remain to obtain atomic-level information in native cell settings for proteins that are small, disordered, or are strongly engaged in intermolecular interactions. In this Minireview, we discuss recent progress in using sensitivity enhanced solid-state NMR spectroscopy methods in the context of cellular structural biology.
Topics & Concepts
ChemistryNuclear magnetic resonance spectroscopyContext (archaeology)Solid-state nuclear magnetic resonanceSpectroscopyIntermolecular forceNanotechnologyBiophysicsChemical physicsNuclear magnetic resonanceMoleculePhysicsStereochemistryMaterials scienceOrganic chemistryPaleontologyQuantum mechanicsBiologyAdvanced Electron Microscopy Techniques and ApplicationsAdvanced NMR Techniques and ApplicationsPhysics of Superconductivity and Magnetism