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Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy

Jelle Folkerts, Nicolas Gaudenzio, Marcus Maurer, Rudi W. Hendriks, Ralph Stadhouders, See‐Ying Tam, Stephen J. Galli

2020Nature Protocols33 citationsDOIOpen Access PDF

Topics & Concepts

DegranulationFunctional genomicsCRISPRBiologyMast cellGene knockdownSmall hairpin RNACell biologyRNA interferenceTransfectionSingle-cell analysisGenome editingComputational biologyConfocal microscopyGene expression profilingCellCell cultureGene expressionGeneGenomicsRNAGeneticsGenomeImmunologyReceptorCRISPR and Genetic EngineeringRNA regulation and diseaseRetinal Development and Disorders
Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy | Litcius