Enzyme cascade-amplified immunoassay based on the nanobody–alkaline phosphatase fusion and MnO<sub>2</sub> nanosheets for the detection of ochratoxin A in coffee
Zeling Zhang, Benchao Su, Huan Xu, Zhenyun He, Yuling Zhou, Qi Chen, Zhichang Sun, Hongmei Cao, Xing Liu
Abstract
. The ECAIA is highly selective for OTA. Good recovery rates (84.3-113%) with a relative standard deviation of 1.3-3% were obtained and confirmed by high performance liquid chromatography with a fluorescence detector. The developed ECAIA was demonstrated to be a useful tool for the detection of OTA in coffee which provides a reference for the analysis of other toxic small molecules.
Topics & Concepts
Ochratoxin AAlkaline phosphataseImmunoassayChemistryEnzymeOchratoxinCascadeNuclear chemistryChromatographyBiochemistryAntibodyFood scienceBiologyMycotoxinImmunologyAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionMonoclonal and Polyclonal Antibodies Research