<p>Downregulation of circRNA_0000285 Suppresses Cervical Cancer Development by Regulating miR197-3p–ELK1 Axis</p>

Abstract

Background: Circular RNAs (circRNAs) are involved in the development of human cancers, including cervical cancer (CC). However, the role and mechanism of the circRNA hsa_circ_0000285 ( circ_0000285 ) in CC development remain largely unknown. Methods: Thirty paired CC and adjacent normal tissue samples were harvested. CC cell lines SiHa and HeLa were cultured in this study. The expression of circ_0000285 , miR197-3p and ELK1 was detected via qRT-PCR or Western blot. CC development was assessed via cell viability, colony formation, apoptosis, cell cycle, and autophagy using MTT, colony-formation assays, flow cytometry and Western blot. The target association was analyzed via dual luciferase–reporter assay, RNA immunoprecipitation, and RNA pull-down. The role of circ_0000285 in CC in vivo was analyzed using a xenograft model. Results: circ_0000285 abundance was enhanced in CC tissue and cells and mainly located in cytoplasm. Silence of circ_0000285 suppressed cell viability and colony formation, arrested the cell cycle at the G 0 /G 1 phase, and induced apoptosis and autophagy in CC cells. miR197-3p was targeted by circ_0000285 , and miR197-3p knockdown reversed the effect of circ_0000285 silence on CC development. miR197-3p directly targeted ELK1 to inhibit CC development. circ_0000285 regulated ELK1 by modulating miR197-3p . Knockdown of circ_0000285 reduced xenograft tumor growth in vivo. Conclusion: Knockdown of circ_0000285 repressed CC development by increasing miR197-3p and decreasing ELK1 . Keywords: cervical cancer, circ_0000285 , miR197-3p , ELK1

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