Lewinella aurantiaca sp. nov., a carotenoid pigment-producing bacterium isolated from surface seawater
Inhyup Kim, Geeta Chhetri, Jiyoun Kim, Minchung Kang, Taegun Seo
Abstract
A Gram-stain-negative, aerobic, rod-shaped, carotenoid-pigmented, motile-by-gliding bacterium, which was designated as SSH13 T , was isolated from a surface seawater sample collected from Sehwa Beach in the Republic of Korea. Strain SSH13 T was oxidase-negative, catalase-positive and grew at 2–37 °C (optimum, 30 °C), in the presence of 0.5–6% NaCl and within a pH range of pH 6–10 (optimum, pH 8). The novel isolate required NaCl for growth and grew optimally with approximately 2 % NaCl. Chemotaxonomic and morphological characteristics were consistent with members of the genus Lewinella . Furthermore, phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain SSH13 T was most closely related to the type strains of the genus Lewinella . Strain SSH13 T had highest 16S rRNA gene sequence similarities to Lewinella persica DSM 23188 T (95.3 %) and Lewinella agarilytica KCTC 12774 T (95.0 %). The major fatty acids of SSH13 T were summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and iso-C 15 : 0 . Strain SSH13 T contained phosphatidylethanolamine as a major polar lipid. Menaquinone-7 was the predominant respiratory quinone. The average nucleotide identity values between strain SSH13 T and L. persica T-3 T and L. agarilytica SST-19 T were 72.9 and 72.6 %, respectively. The DNA G+C content of the genomic DNA was 52.8 mol%. The present study aimed to determine the multiple-antibiotic resistance of the novel bacterium. Based on phylogenetic and phenotypic analyses, strain SSH13 T is considered to represent a novel species of the genus Lewinella , for which the name Lewinella aurantiaca sp. nov. (type strain SSH13 T =KACC 21167 T =NBRC 113866 T ) is proposed.