Targeted pH switched europium complexes monitoring receptor internalisation in living cells
Jack D. Fradgley, Matthieu Starck, Michel Laget, Emmanuel Bourrier, Elodie Dupuis, Laurent Lamarque, Eric Trinquet, Jurriaan M. Zwier, David Parker
Abstract
We report the design and evaluation of pH responsive luminescent europium(iii) probes that allow conjugation to targeting vectors to monitor receptor internalisation in cells. The approach adopted here can be used to tag proteins selectively and to monitor uptake into more acidic organelles, thereby enhancing the performance of time-resolved internalisation assays that require pH monitoring in real time.
Topics & Concepts
EuropiumReceptorChemistryCombinatorial chemistryLuminescenceNanotechnologyBiophysicsCancer researchMaterials scienceBiochemistryMedicineOptoelectronicsBiologyLanthanide and Transition Metal ComplexesMolecular Sensors and Ion DetectionSupramolecular Chemistry and Complexes