Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
Anaïs Oudin, Virginie Baus, Vanessa Barthelemy, Carina Fabian, Eliane Klein, Monika Dieterle, May Wantz, Ann‐Christin Hau, Claire Dording, Amandine Bernard, Alessandro Michelucci, Yahaya A Yabo, Georgia Kanli, Olivier Keunen, Rolf Bjerkvig, Simone P. Niclou, Anna Golebiewska
Abstract
Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing. For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).