Potential of the Signal Peptide Derived from the <i>PAS_chr3_0030</i> Gene Product for Secretory Expression of Valuable Enzymes in <i>Pichia pastoris</i>
Qi Shen, Xiaoting Zhou, Qian Guo, Yu-Zhen Xue, Yu-Zhen Xue, Ya‐Ping Xue, Ya‐Ping Xue, Yu‐Guo Zheng
Abstract
Although P. pastoris is widely used for the secretory production of pharmaceutical proteins, its successful applications in the secretory production of industrial enzymes are limited. The α-mating factor pre-pro leader is the most widely used secretion signal in P. pastoris , but numerous industrial enzymes cannot be secreted using it.
Topics & Concepts
Pichia pastorisSignal peptideEnzymeGeneBiochemistryPeptideProduct (mathematics)Gene productBiologyChemistryGene expressionPeptide sequenceRecombinant DNAGeometryMathematicsNeurobiology and Insect Physiology ResearchProtein Hydrolysis and Bioactive PeptidesCellular transport and secretion