Combined single-cell transcriptional, translational, and genomic profiling reveals HIV-1 reservoir diversity
Gérémy Sannier, Mathieu Dubé, Caroline Dufour, Corentin Richard, Nathalie Brassard, Gloria-Gabrielle Delgado, Amélie Pagliuzza, Amy E. Baxter, Julia Niessl, Elsa Brunet‐Ratnasingham, Roxanne Charlebois, Bertrand Routy, Jean‐Pierre Routy, Rémi Fromentin, Nicolas Chomont, Daniel E. Kaufmann
Abstract
cells, are mostly defective. Although LRAs efficiently induce transcription in all memory cell subsets, we observe induction of translation mostly in effector memory cells, rather than in the long-lived central memory pool. We identify HIV-1 clones with diverse transcriptional and translational patterns between individual cells, and this finding suggests that cell-intrinsic factors influence reservoir persistence and heterogeneity.
Topics & Concepts
BiologyRNATranscription (linguistics)GeneCellEffectorVirus latencyGeneticsComputational biologyVirologyViral replicationCell biologyCell culturePhilosophyLinguisticsHIV Research and TreatmentImmune Cell Function and InteractionT-cell and B-cell Immunology