A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
Kiersten D. Lenz, Shailja Jakhar, Jing W. Chen, Aaron S. Anderson, Dylan C. Purcell, Mohammad O. Ishak, Jennifer Foster Harris, Leyla E. Akhadov, Jessica Z. Kubicek-Sutherland, Pulak Nath, Harshini Mukundan
Abstract
The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need to separate hydrophobic signatures such as lipids from blood. Herein, we present a microfluidic device capable of centrifugal separation of serum from blood at the point of need with a system that is compatible with biomarkers that are both hydrophilic and hydrophobic. The cross-flow filtration device separates serum from blood as efficiently as traditional methods and retains amphiphilic biomarkers in serum for detection.