Exploring the Capabilities of the Penicillium verruculosum Expression System for the Development of Producers of Enzymes for the Effective Degradation of Renewable Plant Biomass: a Review
А. П. Синицын, О. А. Синицына, И. Н. Зоров, А. М. Рожкова
Abstract
This review summarizes the findings on the use of the Penicillium verruculosum В1-537 (ΔniaD) recipient strain for the development of producers of enzymes—β-glucosidase, polysaccharide monooxygenase, and xylanases—that complement the basic cellulase complex and enhance its effectiveness. A special feature of the P. verruculosum strain is the secretion of a basic cellulase complex consisting of cellobiohydrolases and endoglucanases (EGs) that, in terms of their hydrolytic ability, surpass Hypocrea (Trichoderma) strains that are widely used in the laboratory and in industry. The capabilities of the P. verruculosum В1-537 (ΔniaD) expression system can be used to complement the basic cellulase complex with new enzymes, which are required to enhance its hydrolytic activity, and to develop new enzyme preparations tailored to the conversion of different types of renewable plant biomass (RPB). The use of an expression system based on P. verruculosum fungus makes it possible to enhance the ability of basic cellulase complex to convert different types of RPB.