2D Strategy for the Construction of an Enzyme-Activated NIR Fluorophore Suitable for the Visual Sensing and Profiling of Homologous Nitroreductases from Various Bacterial Species
Tao Liu, Yifei Wang, Lei Feng, Xiangge Tian, Jingnan Cui, Zhenlong Yu, Chao Wang, Baojing Zhang, Tony D. James, Xiaochi Ma
Abstract
has been developed to selectively identify NTRs and sensitively determine NTR activity. Using the in-gel assay, NTRs from various bacterial species have been profiled visually from the "fluorescence fingerprints", which facilitates the rapid identification of NTRs from bacterial lysates. Thus, various homologous NTRs were identified from three metronidazole-susceptible bacterial species as well as seven unsusceptible species, which were confirmed by the whole-genome sequence. As such, the evaluation of NTRs from different bacterial species should help improve the rational usage of 5-nitroimidazole drugs as antibiotics.
Topics & Concepts
FluorophoreBacteriaNitroreductaseChemistryEnzymeFluorescenceBiochemistryBiologyGeneticsPhysicsQuantum mechanicsNanoplatforms for cancer theranosticsAdvanced biosensing and bioanalysis techniquesAdvanced Nanomaterials in Catalysis