Engineering of Cyclodextrin Glycosyltransferase through a Size/Polarity Guided Triple-Code Strategy with Enhanced α-Glycosyl Hesperidin Synthesis Ability
Hanchi Chen, Yi Liu, Xiangyi Ren, Jiajun Wang, Linjiang Zhu, Yuele Lu, Xiaolong Chen
Abstract
Size/polarity guided triple-code strategy, a novel semirational mutagenesis strategy, was developed in this study and employed to engineer the aglycon attacking site of CGTase. Screening pressure was set as improved hesperidin glucoside synthesis ability, and eight positive mutants were obtained by screening only 32 mutants.
Topics & Concepts
HesperidinGlycosyltransferaseMutagenesisMutantCyclodextrinChemistryGlycosyl donorPolarity (international relations)Protein engineeringBiochemistryGlycosylationCombinatorial chemistryEnzymeMedicineCellGeneAlternative medicinePathologyEnzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationMicrobial Metabolites in Food Biotechnology