Glycosylated cyclophellitol-derived activity-based probes and inhibitors for cellulases
Casper de Boer, Nicholas G. S. McGregor, Evert Peterse, Sybrin P. Schröder, Bogdan I. Florea, Jianbing Jiang, Jos Reijngoud, Arthur F. J. Ram, Gilles P. van Wezel, Gijsbert A. van der Marel, Jeroen D. C. Codée, Herman S. Overkleeft, G.J. Davies
Abstract
-β-1,4-glucanase. An elaborated activity-based probe facilitated the direct detection and identification of β-1,4-glucanases within a complex fungal secretome without any detectable cross-reactivity with β-d-glucosidases. These probes and inhibitors add valuable new capacity to the growing toolbox of cyclophellitol-derived probes for the activity-based profiling of biomass-degrading enzymes.
Topics & Concepts
CellulaseChemistryEnzymeBiochemistryGlucanaseChromogenicGlucosidasesChromatographyBiofuel production and bioconversionEnzyme Production and CharacterizationPolysaccharides and Plant Cell Walls