Diagnostic assay to assist clinical decisions for unclassified severe combined immune deficiency
Panojot Bifsha, Jennifer W. Leiding, Sung‐Yun Pai, A. Colamartino, Nicholas Hartog, Joseph A. Church, Benjamin Oshrine, Jennifer M. Puck, M. Louise Markert, Élie Haddad
Abstract
Key Points 3D organoid T-cell differentiation from a few hundred peripheral blood CD34+ cells was successfully achieved. 3D organoid T-cell differentiation could help physicians distinguish intrinsic from extrinsic defects underlying a clinical SCID phenotype.
Topics & Concepts
OrganoidImmune systemPhenotypeCD34ImmunologyPeripheral bloodMedicineCellular differentiationBiologyBioinformaticsStem cellNeuroscienceCell biologyGeneticsGeneImmunodeficiency and Autoimmune DisordersBlood disorders and treatmentsCytomegalovirus and herpesvirus research