Gene signature of m6A RNA regulators in diagnosis, prognosis, treatment, and immune microenvironment for cervical cancer
Shizhi Wang, Bo Ding, Shiyuan Wang, Wenjing Yan, Qianqian Xia, Dan Meng, Shuqian Xie, Siyuan Shen, Bingjia Yu, Haohan Liu, Jing Hu, Xing Zhang
Abstract
Abstract Continuing studies imply that m 6 A RNA modification is involved in the development of cervical cancer (CC), but lack strong support on recurrence and diagnosis prediction. In this research, a comprehensive analysis of 33 m 6 A regulators was performed to fulfill them. Here, we performed diagnostic and prognosis models and identified key regulators, respectively. Then the CC patients were separated into two clusters in accordance with 33 regulators, and participants in the cluster 1 had a worse prognosis. Subsequently, the m 6 AScore was calculated to quantify the m 6 A modification pattern based on regulators and we found that patients in cluster 1 had higher m 6 AScore. Afterwards, immune microenvironment, cell infiltration, escape analyses and tumor burden mutation analyses were executed, and results showed that m 6 AScore was correlated with them, but to a limited extent. Interestingly, HLAs and immune checkpoint expression, and immunophenoscore in patients with high-m 6 AScores were significantly lower than those in the low-m 6 AScore group. These suggested the m 6 AScores might be used to predict the feasibility of immunotherapy in patients. Results provided a distinctive perspective on m 6 A modification and theoretical basis for CC diagnosis, prognosis, clinical treatment strategies, and potential mechanism exploration.