Defining steps in RAVE-catalyzed V-ATPase assembly using purified RAVE and V-ATPase subcomplexes
Michael Jaskolka, Maureen Tarsio, Anne M. Smardon, Md. Murad Khan, Patricia M. Kane
Abstract
complexes reconstituted into lipid nanodiscs, consistent with its catalytic activity in vivo. These results provide new insights into the possible order of events in V-ATPase reassembly and the roles of the RAVE complex in each event.
Topics & Concepts
ATPaseV-ATPaseChemistryBiologyEnzymeCell biologyBiochemistryATP Synthase and ATPases ResearchMitochondrial Function and PathologyRNA and protein synthesis mechanisms