Antibody dual-functionalisation enabled through a modular divinylpyrimidine disulfide rebridging strategy
Abigail R. Hanby, Stephen J. Walsh, Andrew J. Counsell, Nicola Ashman, Kim T. Mortensen, Jason S. Carroll, David R. Spring
Abstract
Herein we report the development of a methodology for the dual-functionalisation of IgG antibodies. This is accomplished through the combination of disulfide rebridging divinylpyrimidine technology, with bicyclononyne and methylcyclopropene handles to facilitate sequential SPAAC and IEDDA reactions. Advantageously, the strategy does not require metal catalysis and avoids the need for purification between functionalisation steps.
Topics & Concepts
Disulfide bondModular designCombinatorial chemistryDual (grammatical number)ChemistryCatalysisAntibodyNanotechnologyComputer scienceMaterials scienceBiochemistryBiologyImmunologyArtLiteratureOperating systemMonoclonal and Polyclonal Antibodies ResearchChemical Synthesis and AnalysisClick Chemistry and Applications