Developing a population-state decision system for intelligently reprogramming extracellular electron transfer in <i>Shewanella oneidensis</i>
Feng‐He Li, Qiang Tang, Yangyang Fan, Yang Li, Jie Li, Jing‐Hang Wu, Chen-Fei Luo, Hong Sun, Wen‐Wei Li, Han‐Qing Yu
Abstract
MR-1 could be greatly enhanced by the PSD system via shifting the dominant metabolic flux from initial bacterial growth to subsequent EET enhancement (i.e., after reaching a certain population-state threshold). The strain engineered with this system achieved up to 4.8-fold EET enhancement and exhibited a substantially improved pollutant reduction ability, increasing the reduction efficiencies of methyl orange and hexavalent chromium by 18.8- and 5.5-fold, respectively. Moreover, the PSD system outcompeted the constant expression system in managing EET enhancement, resulting in considerably enhanced electron output and pollutant bioreduction capability. The PSD system provides a powerful tool for intelligently managing extracellular electron transfer and may inspire the development of new-generation smart bioelectrical devices for various applications.