Enzymatic Hydrolysis of Human Milk Oligosaccharides. The Molecular Mechanism of <i>Bifidobacterium Bifidum</i> Lacto-<i>N</i>-biosidase
Irene Cuxart, Joan Coines, Oriol Esquivias, Magda Faijes, Antoni Planas, Xevi Biarnés, Carme Rovira
Abstract
of the acid/base residue, facilitating catalysis. The reaction intermediate of LnbB is best depicted as an oxazolinium ion, with a minor population of neutral oxazoline. The present study sheds light on the processing of oligosaccharides of the early life microbiota and will be useful for the engineering of LnbB and similar glycosidases for biocatalysis.
Topics & Concepts
Bifidobacterium bifidumChemistryBiocatalysisOligosaccharideHydrolysisCatalysisReaction mechanismPopulationEnzyme catalysisEnzymatic hydrolysisEnzymeStereochemistryBifidobacteriumOrganic chemistryLactobacillusDemographySociologyFermentationInfant Nutrition and HealthCarbohydrate Chemistry and SynthesisBreastfeeding Practices and Influences