<i>In vitro</i> study of the blood–brain barrier transport of bioactives from Mediterranean foods
Marta Gallardo-Fernández, Ana Rita Garcia, Ruth Hornedo‐Ortega, Ana M. Troncoso, M. Carmen García-Parrilla, María Alexandra Brito
Abstract
model of the BBB, we assessed its integrity by transendothelial electrical resistance, paracellular permeability and immunocytochemical assays of the adherens junction protein β-catenin. The transport across the BBB was evaluated by ultra-high-performance liquid chromatography high resolution mass spectrometry. Results show that tested bioactives did not impair BBB integrity regardless of the concentration evaluated. Additionally, all of them cross the BBB, with the following percentages: HT (∼70%), TYR (∼50%), TYRS (∼30%), SER (∼30%) and PCA (∼9%). These results provide a basis for the MD neuroprotective role.
Topics & Concepts
TyrosolHydroxytyrosolBlood–brain barrierIn vitroSerotoninMediterranean dietMediterranean climateChemistryBiochemistryBiologyMedicinePolyphenolEcologyInternal medicineAntioxidantEndocrinologyCentral nervous systemReceptorBiochemical Analysis and Sensing TechniquesPhytochemicals and Antioxidant ActivitiesBarrier Structure and Function Studies