Advantages and challenges associated with bisulfite-assisted nanopore direct RNA sequencing for modifications
Aaron M. Fleming, Judy Zhu, Vilhelmina K. Done, Cynthia J. Burrows
Abstract
Nanopore direct RNA sequencing assisted by pseudouridine- and m 5 C-specific bisulfite treatment is a technology that allows sequencing for epitranscriptomic modifications with the possibility of quantitative assessment.
Topics & Concepts
BisulfiteNanopore sequencingNanoporeRNABisulfite sequencingComputational biologyFalse positive paradoxChemistryContext (archaeology)Ribosomal RNAPseudouridineSequence (biology)BiologyDNABiochemistryDNA sequencingGeneTransfer RNANanotechnologyComputer scienceDNA methylationMaterials sciencePaleontologyMachine learningGene expressionRNA modifications and cancerGenomics and Phylogenetic StudiesCancer-related molecular mechanisms research