Mitochondrial respiration analysis in permeabilized porcine left ventricular and human right atrial specimens with ischemia-reperfusion
Chantal Eickelmann, Helmut Raphael Lieder, Sharaf-Eldin Shehada, Matthias Thielmann, Gerd Heusch, Petra Kleinbongard
Abstract
Methods to quantify mitochondrial function in translationally relevant models and in human tissue are needed to improve the translation of cardioprotection to patients. Our present approach, using PMT instead of isolated mitochondria for the quantification of mitochondrial ischemia-reperfusion damage, provides a reference for further studies in translationally relevant large animal models and in human tissue, thus possibly improving the translation of cardioprotection to the benefit of patients with acute myocardial infarction.
Topics & Concepts
IschemiaCardiologyInternal medicineRespirationMedicineAnatomyCardiac Ischemia and ReperfusionCardiac Imaging and DiagnosticsCardiovascular Function and Risk Factors