Site-Specific Labeling of Endogenous Proteins Using CoLDR Chemistry
Rambabu Reddi, Adi Rogel, Efrat Resnick, Ronen Gabizon, Pragati K. Prasad, Neta Gurwicz, Haim Barr, Ziv Shulman, Nir London
Abstract
< 100 nM), which installed a CRBN binder onto BTK. This approach joins very few available labeling strategies that maintain the target protein activity and thus makes an important addition to the toolbox of chemical biology.
Topics & Concepts
ChemistryBruton's tyrosine kinaseCovalent bondProteolysisLigand (biochemistry)Target proteinChemical biologyProtein engineeringBiophysicsFluorescenceBiochemistryChimera (genetics)Combinatorial chemistryComputational biologySignal transductionReceptorEnzymeBiologyTyrosine kinaseGeneOrganic chemistryQuantum mechanicsPhysicsProtein Degradation and InhibitorsPeptidase Inhibition and AnalysisClick Chemistry and Applications