Phase separation and inheritance of repressive chromatin domains

Abstract

Polycomb-associated chromatin and pericentromeric heterochromatin form genomic domains important for the epigenetic regulation of gene expression. Both Polycomb complexes and heterochromatin factors rely on ‘read and write’ mechanisms, which, on their own, are not sufficient to explain the formation and the maintenance of these epigenetic domains. Microscopy has revealed that they form specific nuclear compartments separated from the rest of the genome. Recently, some subunits of these molecular machineries have been shown to undergo phase separation, both in vitro and in vivo , suggesting that phase separation might play important roles in the formation and the function of these two kinds of repressive chromatin. In this review, we will present the recent advances in the field of facultative and constitutive heterochromatin formation and maintenance through phase separation. • Both Polycomb and constitutive heterochromatin components undergo phase separation. • HP1 drives the formation of condensed structures that contribute to silencing function. • Polycomb condensates vary based on the specific paralogs that compose them. • Phase separation properties might contribute to the inheritance of chromatin states

Topics & Concepts