Electrochemiluminescence Immunosensor Based on Nanobody and Au/CaCO<sub>3</sub> Synthesized Using Waste Eggshells for Ultrasensitive Detection of Ochratoxin A
Linzhi Li, Xing Liu, Saijun He, Hongmei Cao, Benchao Su, Tianzeng Huang, Qi Chen, Minghuan Liu, Da‐Peng Yang
Abstract
nanocomposites and nanobody technologies provided an ultrasensitive and highly selective ECL immunosensor for OTA detection in the range of 10 pg/mL-100 ng/mL with a low detection limit of 5.7 pg/mL. Moreover, the as-prepared ECL immunosensor showed excellent performance and high stability. Finally, the proposed ECL sensor was applied to analyze OTA in coffee samples, confirming the desirable accuracy and practical applicability potential. Overall, this work presents a new nanomaterial for fabricating the sensing interface of immunosensors by harnessing natural waste as the source and a method for detecting toxic OTA in foods.
Topics & Concepts
ElectrochemiluminescenceDetection limitOchratoxin AMaterials scienceImmunoassayNanocompositeNanoparticleNanomaterialsNanotechnologyElectrochemistryChemistryChromatographyElectrodeMycotoxinAntibodyPhysical chemistryImmunologyBiologyFood scienceAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionGold and Silver Nanoparticles Synthesis and Applications