Cilo-seq: highly sensitive cell-in-library-out single-cell transcriptome sequencing with digital microfluidics
Qianqian Zhang, Xing Xu, Li Lin, Jian Yang, Na Xing, Xin Chen, Lingling Wu, Jia Song, Chaoyong Yang
Abstract
We described Cilo-seq for high-performance scRNA-seq library construction in a single device with programmed and addressable droplet handling based on digital microfluidics, which allowed simultaneously convenient single-cell isolation, efficient nucleic acid amplification, low-loss nucleic acid purification and high-quality library preparation.
Topics & Concepts
MicrofluidicsNucleic acidDigital microfluidicsDigital polymerase chain reactionComputational biologyNanotechnologyComputer scienceRNAInterface (matter)Identification (biology)CellTranscriptomeBiologyChemistryPolymerase chain reactionMaterials scienceBiochemistryGeneGene expressionElectrodePhysical chemistryGibbs isothermElectrowettingBotanyPulmonary surfactantSingle-cell and spatial transcriptomicsElectrowetting and Microfluidic TechnologiesMicrofluidic and Bio-sensing Technologies