Highly sensitive and rapid detection of hypochlorous acid in aqueous media and its bioimaging in live cells and zebrafish using an ESIPT-driven mycophenolic acid-based fluorescent probe
Prasad M. Sonawane, Neha Jain, Arkaprava Roychaudhury, Su Jeong Park, Vikas K. Bhosale, Mahesh B. Halle, Cheol‐Hee Kim, Satish Balasaheb Nimse, David G. Churchill
Abstract
= 522 nm) and a sub-nanomolar (97.3 nM) detection limit with the detection range of 0 to 50 μM. The potential of Myco-OCl as an excellent biosensor is evident from its successful application for live cell imaging of exogenous and endogenous HOCl. In addition, Myco-OCl enabled us to detect HOCl in a zebrafish inflammatory animal model. These underscore the great potential of Myco-OCl for detecting HOCl in diverse physiological systems. Our findings thus offer a highly promising tool for detecting HOCl in living organisms.
Topics & Concepts
ZebrafishHypochlorous acidChemistryFluorescenceHypochloriteEndogenyMycophenolic acidAqueous mediumAqueous solutionBiochemistryOrganic chemistryMedicineTransplantationQuantum mechanicsSurgeryGenePhysicsNeutrophil, Myeloperoxidase and Oxidative MechanismsNitric Oxide and Endothelin EffectsMolecular Sensors and Ion Detection