A novel and sensitive ratiometric fluorescent quantum dot-based biosensor for alkaline phosphatase detection in biological samples <i>via</i> the inner-filter effect
Penghui Hu, Ruiyan Huang, Ye Xu, Tengfei Li, Jun Yin, Yu Zhu Yang, Liang Yuan, Xiaohan Mao, Li Ding, Chang Shu
Abstract
= 0.9969) with the detection limit of 0.57 U per L. Moreover, the method was successfully applied for detecting the ALP activity in a complex biological matrix (human serum and HepG2 cells) with impressive specificity. In particular, the complicated chemical modifications of QDs and pretreatments of biological samples were not required in the whole detection procedures. Therefore, it not only provided a sensitive, specific and simple approach to clinical ALP activity detection, but it also provided support for early diagnosis of diseases.
Topics & Concepts
FluorescenceQuantum dotBiosensorAlkaline phosphataseChemistryNanotechnologyMaterials scienceBiochemistryPhysicsEnzymeOpticsCarbon and Quantum Dots ApplicationsAdvanced biosensing and bioanalysis techniquesQuantum Dots Synthesis And Properties