Genetic polymorphisms in <i>interleukin-6</i> and <i>interleukin-1-beta</i> were associated with dental caries and gingivitis
Caio Luiz Bitencourt Reis, Mariane Carolina Faria Barbosa, Bárbara Maria de Souza Moreira Machado, Samantha Schaffer Pugsley Baratto, Daniela Coêlho de Lima, Aleysson Olímpio Paza, Flares Baratto‐Filho, João Armando Brancher, Érika Calvano Küchler, Daniela Silva Barroso de Oliveira
Abstract
Objective Evaluate the association between single nucleotide polymorphisms (SNPs) in Interleukin-6 (IL-6) gene (rs1800795) and in Interleukin-1-beta (IL-1β) gene (rs1143627 and rs1143629) with dental caries and gingivitis in Brazilian children.Material and methods Three hundred and fifty-three children aged 8–11 years were included. Visible biofilm and gingival bleeding were evaluated by Community Periodontal Index. The International System for Detection and Assessment of Carious Lesions (ICDAS) was used to investigate dental caries. Real-time PCR evaluated SNPs in the DNA. Chi-square test, haplotype analysis and logistic regression were applied (alpha of 5%).Results The GG genotype in rs1800795 (IL-6) decreases the risk of gingivitis in a co-dominant model (p = .05; OR = 0.64). The GG genotype in rs1143627 (IL-1β) reduces the risk of dental caries (Co-dominant model: ICDAS0 versus ICDAS1-6: p = .05; OR = 0.55. ICDAS0-2 versus ICDAS3-6: p = .02; OR = 0.49. Recessive model: ICDAS0 versus ICDAS1-6: p = .005; OR = 0.48. ICDAS0-2 versus ICDAS3-6: p = .004; OR = 0.45. Logistic regression: ICDAS0-2 versus ICDAS3-6: p = .05; OR = 0.24; CI 95%= 0.05–1.00). The GG genotype in rs1143629 was more frequent in ICDAS0 (p = .05; OR: 0.60). In the haplotype analysis, IL-1β was associated with gingivitis.Conclusion The rs1800795 in IL-6 gene was associated with gingivitis. The rs1143627 and rs1143629 in IL-1β were associated with dental caries and gingivitis.