A novel chitosan–PEG hydrogel embedded with <i>in situ</i> silver nanoparticles of <i>Clerodendrum glandulosum</i> Lindl. extract: evaluation of its <i>in vivo</i> diabetic wound healing properties using an image-guided machine learning model
Ankit Majie, Rajdeep Saha, Arnab Sarkar, Rudranil Bhowmik, Sanmoy Karmakar, Vishal Sharma, Kaushal Deokar, Asad ul Haque, Sanjaya Shankar Tripathy, Biswatrish Sarkar
Abstract
(CG) extract reduced silver nanoparticle (AgNP) impregnated chitosan-polyethylene glycol (PEG) hydrogel was synthesized. The findings of the physicochemical characterization studies suggested that the hydrogel exhibited excellent formulation characteristics and showed controlled release for seven days, making it suitable for chronic wound healing studies. In subsequent studies, these formulations showed good antioxidant and antimicrobial properties, and hemocompatibility, with the least cytotoxic properties. The results of the diabetic wound healing studies showed a faster wound closure rate and improved extracellular matrix formation. These antioxidant, antimicrobial, anti-inflammatory and wound-healing properties suggest that the CG-AgNP loaded chitosan-PEG hydrogel is a promising material for novel topical formulation of diabetic wounds.