Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method
Martha Maggira, Ioannis Sakaridis, Maria Ioannidou, Georgios Samouris
Abstract
Various analytical techniques for detecting mycotoxins have been developed in order to control their concentration in food and feed. Conventional analytical approaches for mycotoxin identification include thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and gas chromatography (GC). Rapid methods for mycotoxin analysis are also becoming increasingly relevant. One of the most common rapid methods for determining these compounds is the enzyme-linked immunosorbent assay (ELISA). The current study aimed to compare three available ELISA kits for the detection and quantification of aflatoxins B1, B2, G1, and G2 in spiked feed samples at known quantities. All three ELISA kits were validated and showed good performance with high recovery rates and LOD and LOQ values lower than the MRL. The developed HPLC-FL method was validated for all the compounds determining the accuracy, precision, linearity, decision limit, and detection capability with fairly good results. Unknown feed samples (corn, silage, pellet, barley, wheat, soya, and sunflower) were also tested using the best ELISA kit and HPLC, and the results were compared. Both ELISA and HPLC were proven to be suitable methods for mycotoxin analysis. The analytical technique should be determined primarily by the availability and number of samples.