Polarimetric second-harmonic generation microscopy of the hierarchical structure of collagen in stage I-III non-small cell lung carcinoma
Ahmad Golaraei, Leila B. Mostaço-Guidolin, Vaishnavi Raja, Roya Navab, Tao Wang, Shingo Sakashita, Kazuhiro Yasufuku, Ming‐Sound Tsao, Brian C. Wilson, Virginijus Barzda
Abstract
Polarimetric second-harmonic generation (P-SHG) microscopy is used to quantify the structural alteration of collagen in stage-I,-II and -III non-small cell lung carcinoma (NSCLC) ex vivo tissue. The achiral and chiral molecular second-order susceptibility tensor components ratios ( R and C , respectively), the degree of linear polarization ( DLP ) and the in-plane collagen fiber orientation ( δ ) were extracted. Further, texture analysis was performed on the SHG intensity, R , C , DLP and δ . The distributions of R , C , DLP and δ as well as the textural features of entropy, correlation and contrast show significant differences between normal and tumor tissues.