Lipid Membrane Interaction of Peptide/DNA Complexes Designed for Gene Delivery
Neval Yilmaz, Yutaka Kodama, Keiji Numata
Abstract
oxidase subunit IV (Cytcox) and BP100, which are a mitochondria-targeting signal peptide and a CPP, respectively. QCM data showed that BP100 has a higher binding affinity than Cytcox to both plasma membrane- and mitochondrial membrane-mimicking lipid bilayers. The DNA complexes with either Cytcox or BP100 exhibited the same tendency. Furthermore, HS-AFM data demonstrated that the DNA complexes of either peptide can disrupt the lipid membranes, forming larger pores in the case of Cytcox. Our results suggest that the binding affinity of the peptide/DNA complex to the plasma membrane is more critical than its membrane disruption ability in enhancing the cellular uptake of DNA.
Topics & Concepts
MembranePeptideBiophysicsChemistryDNAQuartz crystal microbalanceBiochemistryLipid bilayerLiposomeBiologyOrganic chemistryAdsorptionRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesLipid Membrane Structure and Behavior