Naive stem cell blastocyst model captures human embryo lineage segregation
Ayaka Yanagida, Daniel Spindlow, Jennifer Nichols, Anish Dattani, Austin Smith, Ge Guo
Abstract
Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research.
Topics & Concepts
BiologyBlastocystEmbryoLineage (genetic)Cell biologyStem cellInner cell massAndrologyCell lineageEvolutionary biologyGeneticsCellular differentiationEmbryogenesisGeneMedicineSingle-cell and spatial transcriptomicsPluripotent Stem Cells ResearchCancer Genomics and Diagnostics