Effect of dielectric barrier discharge (DBD) plasma on the structure and antioxidant activity of bovine serum albumin (BSA)
Zhiwei Liu, Muhammad Faisal Manzoor, Yi‐Cheng Tan, Muhammad Inam‐Ur‐Raheem, Rana Muhammad Aadil
Abstract
Abstract This study was aimed to determine the influence of dielectric barrier discharge (DBD) plasma with a plasma intensity (20 and 40 kV) and treatment times (0, 2, 4, 6 and 8 min) on the physicochemical, structural and antioxidant properties of bovine serum albumin (BSA) solution. In results, the free sulphydryl content, pH and free amino group contents, while carbonyl groups and surface hydrophobicity were increased after the plasma treatment. After plasma treatment, there is also an increase in the DPPH and FRAP assay that was associated with the changes in the structure of the peptides chain. The complementary techniques were employed to identify the protein structural modifications by plasma treatment. These changes were more severe at the most extended treatment duration and plasma intensity. The high energetic and oxidising species carried by plasma may have significant associations on the structural and physical properties of BSA.