Expanding the functionality of proteins with genetically encoded dibenzo[<i>b</i>,<i>f</i>][1,4,5]thiadiazepine: a photo-transducer for photo-click decoration
Qin Xiong, Tingting Zheng, Xin Shen, Baolin Li, Jielin Fu, Xiaohu Zhao, Chunxia Wang, Zhipeng Yu
Abstract
Via directed evolution of the tRNA synthetase, genetic encoding of a unique DBTD derived ncAA into proteins is realized. The DBTD residue is capable of transducing photon energy into ring-strain energy in situ for photo-clicking with diarylsydnone.
Topics & Concepts
TransducerClick chemistryComputational biologyComputer scienceChemistryBiologyCombinatorial chemistryEngineeringElectrical engineeringClick Chemistry and ApplicationsPhotochromic and Fluorescence ChemistryChemical Synthesis and Analysis