Silencing UCHL3 enhances radio-sensitivity of non-small cell lung cancer cells by inhibiting DNA repair
Miaowen Liu, Huimin Chen, Xinyue Chen, Jianping Xiong, Zhiwang Song
Abstract
. Furthermore, γH2AX foci staining and Western blot analysis showed that knockdown of UCHL3 increased IR-induced DNA damage. Knockdown of UCHL3 in NSCLC cells decreased homologous recombination (HR) repair efficiency and RAD51 foci formation. Collectively, our study revealed that knockdown of UCHL3 enhanced the radio-sensitivity of NSCLC cells and increased IR-induced DNA damage via impairing HR repair.
Topics & Concepts
Gene knockdownRAD51DNA damageCancer researchDNA repairGene silencingLung cancerBiologyMolecular biologyCell cultureChemistryDNAMedicinePathologyGeneticsGeneDNA Repair MechanismsRadiation Therapy and DosimetryPARP inhibition in cancer therapy