The human mitochondrial enzyme BCO2 exhibits catalytic activity toward carotenoids and apocarotenoids
Linda D. Thomas, Sepalika Bandara, Vipul M. Parmar, Srinivasagan Ramkumar, Nimesh Khadka, Marcin Golczak, Philip D. Kiser, Johannes von Lintig
Abstract
did not impede the chimeric enzyme's catalytic proficiency. We further showed that the chimeric BCO2 displayed broad substrate specificity and converted carotenoids into two ionones and a central C14-apocarotendial by oxidative cleavage reactions at C9,C10 and C9',C10'. Thus, our study demonstrates that human BCO2 is a catalytically competent enzyme. Consequently, information on BCO2 becomes broadly applicable in human biology with important implications for the physiology of the eyes and other tissues.
Topics & Concepts
BiochemistryCarotenoidBiologyOxygenaseEnzymeDioxygenasePeptide sequenceGeneEscherichia coliGeneticsAntioxidant Activity and Oxidative StressRetinoids in leukemia and cellular processesFree Radicals and Antioxidants