Multistep Metabolic Engineering of Bacillus licheniformis To Improve Pulcherriminic Acid Production
Shi‐Yi Wang, Huan Wang, Dan Zhang, Xiaoyun Li, Jiang Zhu, Yangyang Zhan, Dongbo Cai, Qin Wang, Xin Ma, Dong Wang, Shouwen Chen
Abstract
Pulcherriminic acid is a cyclodipeptide derived from cyclo( l -Leu– l -Leu), which shares the same iron chelation group with hydroxamate sidephores. Generally, pulcherriminic acid-producing strains could be the perfect candidates for antibacterial and anti-plant-pathogenic fungal agents. In this study, we obtained the promising W4/pHY- yvmA pulcherriminic acid-producing strain via a multistep metabolic modification. The engineered W4/pHY- yvmA strain is able to achieve 556.1 mg/liter pulcherriminic acid production, which is the highest yield so far to the best of our knowledge.
Topics & Concepts
Bacillus licheniformisMetabolic engineeringBiochemistryOperonStrain (injury)BiosynthesisYield (engineering)BiologyChemistryGeneBacteriaMutantBacillus subtilisAnatomyMetallurgyMaterials scienceGeneticsRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyGenomics and Phylogenetic Studies